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[Study on the interface of human hepatocyte L-02 polypropylene:simple culture method of human hepatocyte with spheroidal aggregate culture].
Peng, Cheng-hong; Han, Bao-san; Gao, Chang-you; Ma, Zu-wei; Zhao, Zhi-ming; Wang, Yong; Liu, Hong; Zhang, Gui-di; Yang, Mei-juan.
Affiliation
  • Peng CH; Department of General Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Zhonghua Wai Ke Za Zhi ; 42(17): 1064-8, 2004 Sep 07.
Article in Zh | MEDLINE | ID: mdl-15498321
OBJECTIVE: To found new interface of human hepatocyte/poly propylene with good cytocompatibility for made polypropylene hollow fibers bioreactor of bioartificial liver in future. METHODS: Using the macromolecular hydroperoxide groups on the polypropylene membrane surface as initiators, acrylamides were polymerized on the polypropylene membranes, under induction by both UV irradiation and Fe2+ reduction. Growth characteristics of human hepatocyte L-02 were detected when it was cultured on polystyrene, polypropylene and modified polypropylene membrane surface. RESULTS: Water contact angle measurement of the polypropylene and the modified polypropylene membranes decreased from (72 +/- 5) degrees to (30 +/- 4) degrees , which indicated that the hydrophilicity of the membrane was improved obviously after the grafting modification. Human hepatocyte L-02 could not adhere and spread on modified polypropylene membrane surface, and grown in spheroidal aggregate with higher density and higher proliferation ratio measured by MTT method. CONCLUSIONS: Acrylamide polymerized on the polypropylene membranes is a good method which not only improved human hepatocytes cytocompatibility but also found a new simple culture method with spheroidal aggregate culture of human hepatocyte.
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Collection: 01-internacional Database: MEDLINE Main subject: Polypropylenes / Cell Culture Techniques / Hepatocytes Limits: Humans Language: Zh Journal: Zhonghua Wai Ke Za Zhi Year: 2004 Document type: Article Affiliation country: Country of publication:
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Collection: 01-internacional Database: MEDLINE Main subject: Polypropylenes / Cell Culture Techniques / Hepatocytes Limits: Humans Language: Zh Journal: Zhonghua Wai Ke Za Zhi Year: 2004 Document type: Article Affiliation country: Country of publication: